Table of contents
No headers
#1: Transposon Mutagenesis Insertion Sequences
Brad Goodner June 8, 2010:
Below are some pdf files on the construction of the transposon we used in the Institute (Larsen et al), hints for using the EPICENTRE transformation-version of the transposon (Helpful hints for using transposomes), and a recent paper on a modified version of the transposon for use in Bacillus and hopefully other Gram positive bacteria (EZTn5 custom transposon for Bacillus).
Helpful hints for using Transposomes.pdf
EZ-Tn5 custom transposome B subtilis.pdf
Brad Goodner June 8, 2010:
Below is the sequence of pRL27, the plasmid carrying Tn5-RL27 which is the transposon we used in the Institute.
Transposon Plasmid pRL27 Sequence.doc
Brad Goodner June 9, 2010:
Below is a PowerPoint poster-size file with the most common microbiology lab tests.
Brad Goodner June 10, 2010:
Below is the pdf of the paper from Roberto Kolter's lab that describes a plate-based assay for biofilm mutants as well as several other biofilm methods that are useful.
- Tag page
- What links here
Files 7
| File | Size | Date | Attached by | |||
|---|---|---|---|---|---|---|
 | TransposonMutagenesisInsertionSequences.doc No description | 37.5 kB | 11:12, 8 Jun 2010 | kdaley | Actions | |
| Larsen_et_al.pdf No description | 689.6 kB | 13:37, 8 Jun 2010 | goodnerbw | Actions | |
| Helpful hints for using Transposomes.pdf No description | 88.02 kB | 13:37, 8 Jun 2010 | goodnerbw | Actions | |
| EZ-Tn5 custom transposome B subtilis.pdf No description | 741.56 kB | 13:37, 8 Jun 2010 | goodnerbw | Actions | |
| Transposon Plasmid pRL27 Sequence.doc No description | 27.5 kB | 13:56, 8 Jun 2010 | goodnerbw | Actions | |
| testposter.ppt No description | 1349.5 kB | 00:00, 10 Jun 2010 | goodnerbw | Actions | |
| PseudomonasBiofilmMutants.pdf No description | 512.33 kB | 00:21, 10 Jun 2010 | goodnerbw | Actions | |